董常生，王海东，姜俊兵，贺俊平，赫晓燕 (山西农业大学动物科技学院，山西太谷，030801)

摘 要：实验通过克隆分析羊驼催乳素基因的部分序列，对羊驼催乳素基因的结构和功能进行初步探索和揭示。从GENEBANK中已报到的脊椎动物催乳素基因保守区设计一对引物，采用Trizol法提取羊驼胎盘总RNA，利用RT-PCR技术扩增出长度约为510bp的片断。测序后在NCBI工作平台中进行BLASTn同源性比较，得出结论：羊驼催乳素基因与已登录的哺乳动物催乳素基因同源性均超过85%，最高达97%。借助DNAstar分子生物学分析软件绘制了相关动物的遗传进化图，并对羊驼的种属地位进行了进一步验证。

Molecular Cloning and Analysis of Alpaca Prolactin Gene Sequences

Dong Changsheng, Wang Haidong, Jiang Junbing, He Junping,He Xiaoyan (College of Animal science and veterinary medicine,Shanxi Agricultural University,Taigu,030801) Abstrct: By cloning and analysis the partial sequence of alpaca prolactin(PRL) The purpose of this research is to qust for framework and function.According to conservative domain of the published sequence of prolactin gene of vertebrate in GENEBANK, a pair of primers that can amplify alpaca PRL gene was designed and synthesized. The tatol RNA was extracted by Trizol.The fragments cDNA about 510 bp was amplyfied by RT-PCR.The sequence was compared with published PRL genes in Genebank by BLASTn, the sequence showed hightest homology with PRL gene.All homology are over 85% and the highest is 97%.In virtue of DNAstar molecular biology analysis software, phylogenetic tree of different species animal PRL gene was protracted. 篇目13 Morpho蝴蝶结构显色特性研究 刘广平，宣益民，韩玉阁 (南京理工大学动力学院， 江苏 南京 210094) 摘要：Morpho 蝴蝶结构显色是蝴蝶显色研究中最热门的分支之一，其显色原理主要在于物理光学作用。从Morpho蝴蝶的表皮微结构出发，说明了其结构显色现象，并对表皮微结构建立计算模型；利用时域有限差分方法(FDTD)对结构模型在自然光照射下的结构显色特性进行计算，计算分析结果与实验观察现象完全吻合,并根据计算结果分析其结构显色的根本机理，揭示了Morpho 蝴蝶显示亮蓝色的显色机理，说明研究方法具有可行性。 Structure-color Characteristic of Morpho butterfly LIU Guang-ping，XUAN Yi-min，HAN Yu-ge (School of Power Engineering NUST, Nanjing 210094, Jiangsu, China)