|
|
用组织光学特性鉴别诊断人离体的正常膀胱和膀胱癌组织
激光生物学报摘要 2006-5
|
许静芬1 , 魏华江2*, 巫国勇3, 何博华4, 张 薇1
(1. 广东药学院电子物理学教研室, 广东 广州 510006; 2. 华南师范大学激光生命科学研究所、暨激光生命科学教育部重点实验室, 广东 广州 510631; 3. 中山大学第一附属医院, 广东 广州 510080; 4. 广东药学院外科教研室, 广东 广州 510224)
摘要： 研究正常人膀胱和膀胱癌组织在Kubelka-Munk二流模型下对476.5 nm, 514.5 nm和808 nm波长的激光的光学特性的差异。采用双积分球系统和Kubelka-Munk二流模型进行测量研究。实验结果表明，正常膀胱和膀胱癌组织在Kubelka-Munk二流模型下对476.5 nm, 514.5 nm和808 nm波长的每一个波长的激光的吸收、散射、总衰减、有效衰减系数都有非常显著性的差异 (P﹤0.01)。膀胱癌组织对476.5 nm, 514.5 nm和808 nm波长的激光的吸收系数明显地较正常膀胱组织对相应波长的激光的吸收系数要大 (P﹤0.01)，膀胱癌组织对476.5 nm和514.5 nm波长的激光的散射系数明显地较正常膀胱组织对相应波长的激光的散射系数要小(P﹤0.01)，而膀胱癌组织对808 nm波长的激光的散射系数明显地较正常膀胱组织对同一波长的激光的散射系数要大(P﹤0.01)。膀胱癌组织对476.5 nm, 514 nm.5和808 nm波长的激光的总衰减系数明显地较正常膀胱组织对相应波长的激光的总衰减系数要大(P﹤0.01)，膀胱癌组织对476.5 nm, 514.5 nm和808 nm波长的激光的有效衰减系数明显地较正常膀胱组织对相应波长的激光的有效衰减系数要大(P﹤0.01)。提示使用双积分球系统和Kubelka-Munk二流模型来确定离体的正常膀胱组织和膀胱癌组织对476.5 nm, 514.5 nm和808 nm波长的激光的光学特性的差异鉴别诊断病变的膀胱组织是一个有效的方法。
|
|
Differential Diagnosis of Human Normal Bladder and Bladder Cancer Tissues by Utilizing Optical Properties of Tissues in vitro
XU Jing-fen1, WEI Hua-jiang2*, WU Guo-yong3, HE Bo-hua4, ZHANG Wei1
(1. Department of Electronic & Physics, Guangdong Pharmaceutical University, Guangzhou510006, Guangdong, China; 2. MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510631, Guangdong, China; 3. Affiliated Hospital of Sun-Yat-Sen University, Guangzhou 510080, Guangdong, China; 4. Department of Surgery, Guangdong Pharmaceutical University, Guangzhou510006, Guangdong, China)
Abstract：Difference of optical properties of human normal bladder and human bladder cancer tissues at 476.5 nm, 514.5 nm and 808 nm radiation respectively in Kubelka-Munk two-flux model was studied. A double-integrating-spheres system and Kubelka-Munk two-flux model were used for the study. The results of experiment showed that there were very significant difference for the absorption, scattering, total attenuation, effective attenuation coefficients of human normal bladder and bladder cancer tissues at each laser wavelength of 476.5 nm, 514.5 nm and 808 nm radiation respectively in Kubelka-Munk two-flux model (P﹤0.01). Absorption coefficients of human bladder cancer tissue at 476.5 nm, 514.5 nm and 808 nm radiation respectively were obviously bigger than absorption coefficients of human normal bladder tissue at the same wavelength as that wavelength of radiating human bladder cancer tissue(P﹤0.01). Scattering coefficients of human bladder cancer tissue at 476.5 nm and 514.5 nm radiation respectively were obviously smaller than scattering coefficients of human normal bladder tissue at the same wavelength as that wavelength of radiating human bladder cancer tissue (P﹤0.01), and scattering coefficient of human bladder cancer tissue at 808 nm radiation were obviously bigger than scattering coefficient of human normal bladder tissue at the same wavelength (P﹤0.01). Total attenuation coefficients of human bladder cancer tissue at 476.5 nm, 514.5 nm and 808 nm radiation respectively were obviously bigger than total attenuation coefficients of human normal bladder tissue at the same wavelength as that wavelength of radiating human bladder cancer tissue(P﹤0.01). Effective attenuation coefficients of human bladder cancer tissue at 476.5 nm, 514.5 nm and 808 nm radiation respectively were obviously bigger than effective attenuation coefficients of human normal bladder tissue at the same wavelength as that wavelength of radiating human bladder cancer tissue (P﹤0.01). The results imply that it is a effective method to differential diagnosis of pathological bladder tissues by using a double-integrating-spheres system and Kubelka-Munk two-flux model by determining difference of optical properties of human normal bladder and bladder cancer tissues at 476.5 nm, 514.5 nm and 808 nm laser wavelengths respectively in vitro.
|
|
|
|
|
|
Bioinformatics,
sequence analysis; GCG; Life Science News; Drug Discovery.
|
|
