李洁, 王志方, 彭巧铃, 聂刘旺*
（安徽师范大学生命科学学院，安徽 芜湖241000）
摘 要：Dmrt基因家族是一个与性别决定相关的基因家族。该家族成员都含有一个具有DNA结合能力的保守基序――DM结构域，在性别决定和分化发育的调控中担负着重要的功能。本文采用简并PCR技术，扩增和克隆了山地麻蜥(Eremias breuchleyi)基因组中的DM结构域，通过SSCP技术筛选和测序得到了7个具有不同DM序列的克隆。结果显示，在山地麻蜥基因组中存在着Dmrt基因家族的多个成员，与其他动物相关的Dmrt基因进行聚类分析，显示该基因家族在动物系统进化上具有高度的保守性。
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Sequence Analysis of Seven Dmrt Genes in Lacertid Lizards,Eremias breuchleyi
LI jie, WANG zhi-fang, PENG qiao-ling, NIE liu-wang*
(Life Science College, Anhui Normal University, Wuhu 241000, Anhui, China)
Abstract: The Dmrt genes constitute a new gene family related to sex-determination. They encoded transcription factors characterized by a conserved zinc finger-like DNA-binding motif, the DM domain. In this paper, degenerate PCR was used to amplify the conserved DM domains of Dmrt genes in Eremias breuchleyi. Then , seven different DM sequences were identified and named as EbDmrt1a, EbDmrt1b, EbDmrt1c, EbDmrt1d, EbDmrt3, EbDmrt4 and EbDmrt5 according to its name Eremias breuchleyi. The results indicated that Dmrt genes were highly conservative in phylogeny and have several members.
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