PCR-SSCP Analysis on Heart Fatty Acid-Binding Protein Gene in Pigs
YANG Wen-ping1，XUE Shang-jun2，WANG Zhi-wu3，LIU Jian-hua1，
CAO Guo-qing1， ZHOU Zhong-xiao1*
(1. College of Animal Science ，Shanxi Agricultural University，Taigu 030801，China；
2. Da Tong Pig Breeding Farm，Datong 037000，China；
3.Institute of Animal Science and Veterinary Medicine，Shanxi Academy of Agricultural Sciences，Taiyuan 030032，China)
Abstract：The polymorphism of H-FABP gene in Shanxi White pig，Mashen pig，Large White pig，Landrace and Duroc was analyzed by PCR-SSCP. The results indicated that one polymorphic site was discovered in the first intron of porcine H-FABP gene. It had two alleles，designated A and B. Frequency of BB genotype was the highest，and frequency of B allele was obviously higher than frequency of A allele in mashen pig. Frequency of AA genotype was the highest in other breeds. Sequence analysis revealed that this site was generated because of C→T transition.
