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绿 僵 菌 Ma83 几 丁 质 酶 的 发 酵 研 究*
激光生物学报摘要 2006-6
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蔡荟梅1,2,3 , 刘 斌2,蔡敬民2, 吴 克2, 樊美珍3
（1．安徽省产品质量监督检验所，安徽 合肥230022；2合肥学院化学与生物工程系，合肥230022；3安徽农业大学虫生真菌研究所，安徽 合肥230036）
摘 要：本实验从虫生真菌中筛选出金龟子绿僵菌Ma83菌株，它的几丁质酶合成能力最强。其产酶的适宜条件是，碳源为胶体几丁质加葡萄糖，氮源为NaNO3，培养温度为28℃，培养基起始pH6.0；接种量为5ml液态种，最适装液量为5ml ，添加维生素C可以提高酶活；正交实验表明培养因子的最佳组合是：NaNO3 1g/L,胶体几丁质0.6g/L,酵母膏0.05g/L,葡萄糖0.10g/L。根据液态培养产酶过程结果可知，当Ma83菌培养6天时，几丁质酶活力达到8.1U/ml。
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Studies on Fermentation Conditions of Chitinase Produced by Metarhizium anisopliae Ma83
CAI Hui-mei1，2，3, LIU Bin1, CAI Jing-min1, WU Ke1, FAN Mei-zhen3
(1．Department. of Chemistry and Biotechnology, Hefei Union University,Hefei,230022； 2．Anhui Products Quality Supervision And Inspection Institute, 230022；3．Institute of Entomogenous Fungi, Anhui Agriculture University, Hefei, 230036）
Abstract：Strain Ma83 of M.anisopliae with high chitinase activity was selected from strains of entomogenous fungi . The result of the studies on conditions of chitinase production of strain Ma83 in liquid fermentation showed that colloidal chitin and glucose were the best carbon sources. NaNO3 was optimum nitrogen source. Initial pH at 6.0 and temperature of 28℃ were the most suitable conditions for chitinase production . Addition of Vitamin C increased chitinase synthesis. Orthogonal experiment show that the optimal media composition was NaNO3 1g/L, colloidal chitin 0.6g/L, yeast extract 0.05g/L, Glucose 0.10g/L Under optimal conditions, maximum chitinase activity reached 8.1U/ml after being cultured for 6 days .
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Bioinformatics,
sequence analysis; GCG; Life Science News; Drug Discovery.
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