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Isolation and 16 S rDNA Sequence Analysis of Degrading Methionine Bacterium from Ruminal Fluid
LI Lǘ-mu , DIAO Huan , HU Liang-wei , LING Jun
(College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036 , Anhui , China)
Abstract: In order to study species resource from ruminal fluid, one strain of methionine- degrading bacterium MB6-1 was isolated and purified. 16 S rRNA encoding gene (16 S rDNA) from MB6-1 was amplified by PCR, and its nucleotide sequence was determined. On the basis of the homology and phylogenetic analysis of 16 S rDNA（ribosomal database projectⅡ，RDPⅡ）, it was considered that strain MB6-1 may be a novel species of the Providencia family. The GenBank accession numbers of the 16 S rDNA sequences of strain MB6-1 is DQ436917.
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