王现伟1，2 , 胡 林1，许锋林1，刘艳辉1
（1.贵州大学理学院，光电子技术与应用省级重点实验室，贵州 贵阳550025 ；2. 新乡医学院生命科学技术系，河南 新乡453003）
摘 要：目的：通过研究红细胞膜流动性以及红细胞骨架结构的改变，进一步探讨高脂血症大鼠红细胞变形能力改变的机制。方法： 16只Wistar大鼠随机分为两组：高血症组和对照组。高脂组给予高脂饮食。16周后, 腹主动脉采血，采用酶比色法检测血浆甘油三脂、胆固醇含量；并利用激光衍射法测定红细胞变形指数、取向指数，荧光偏振法测定红细胞膜流动性, 激光共聚焦显微镜观测红细胞骨架改变和红细胞F-actin的含量。结果 ：发现高脂血症大鼠红细胞的变形指数、取向指数以及红细胞膜的流动性显著降低（P <0.05），红细胞形态和骨架发生改变，F-actin含量显著降低（P <0.05）。结论： 高脂血症大鼠红细胞变形能力降低与红细胞膜结构改变有一定的关系。
|
|
Studies on the Changes of Erythrocyte Deformability and it's Mechanisms
in Rats with Hyperlipidemia with Laser Biology Techniques
WANG Xian-wei1,2, HU Lin1 , XU Feng1 , LIU Yan-hui1
（1. College of Sciences of Guizhou University, Photoelectron Technology and Application Laboratory, Guiyang 550025 , Guizhou , China ; 2 .Department of Life Science and Technology of Xinxiang Medical College, Xinxiang 453003, Henan , China )
Abstract: Objective：To explore the changes of erythrocyte deformability and possible mechanisms of rats suffering from hyperlipidemia induced by high-fat diet. Methods ：Sixteen rats were randomly divided into two groups: the control group and the hyperlipidemia group. The control group animals were fed with standard laboratory chow; the hyperlipidemia ones were fed with high-fat diet. Sixteen weeks later, blood was draw by abdominal aortic puncture, anti-coagulated with heparin. Then the plasma TG and Ch, erythrocyte deformation index, orientation index, and membrane fluidity were measured; and erythrocyte skeleton was observed with a laser confocal microscopy. Results：Compared with the control group, deformation index, orientation index, and membrane fluidity decreased significantly in rats with hyperlipidemia（P<0.05）; moreover, the integrity of cytoskeleton was destroyed and the contents of F-actin reduced significantly（P<0.05） as well. Conclusion：
|
