激光生物学报摘要, 更新时间: 2007年4月29日
  由美国生科集团 (BVTech, Inc.) 主办
  
XeCl 准分子激光对少根根霉的生物学效应和脂肪酶活性的相关性
激光生物学报摘要 2007-2

郑 敏1,吴 克1,潘仁瑞1,姚尚华1,刘 斌1,蔡敬民2* (1.合肥学院生物与环境工程系,安徽 合肥230022; (2.中国科学院安徽光学精密机械研究所,中国安徽 合肥230031)

摘 要: 利用XeCl准分子紫外激光照射少根根霉孢囊孢子,研究其生物学效应。在照射功率为2.0 kV,2.5 kV和2.7 kV,脉冲次数为35~135的范围内,其致死曲线在85脉冲次数处形成高峰,在110脉冲次数处形成低谷,构成一个“峰形”曲线;与紫外灯UV致死曲线有较大的差别。通过琼脂平板分离、固态发酵,从中筛选到8株脂肪酶活力提高幅度10 % 以上的菌株,其中以突变株LB-5的酶活力最高,达66.4 IU/g干基,较出发菌株酶活提高了55.1 %。讨论了激光照射功率、脉冲次数和致死率与根霉脂肪酶活力之间的关系。


Biological Effect of XeCl Excimer Laser on Rhizopus arrhizus and Its Relationship of Lipase Activity

ZHENG Min1,WU Ke1,PAN Ren-rui1,YAO Shang-hua1,LIU Bin1, CHEN Yong-rong2,CAI Jing-min*1 (1.Department of Biological and Environmental,Hefei University, Hefei 230022,Anhui,China; 2.Anhui Institute of Optics & Fine Mechanics, Chinese Academy of Sciences,Hefei 230031,Anhui,China)

Abstact: The biological effect of R.arrhizus was studied by utilizing XeCl excimer laser in condition of radiation power being 2.0 Kv,2.5 Kv and 2.7 Kv,pulse number ranging between 35~135. A peaky lethel curve was found when flood tide was formed at 85 pulse number and low tide was formed at 110 pulse number,which was much distinctively to the lethel curve caused by UV lamp radiation.8 strains were selected through agar flat separation and solid state ferment.their lipase actives were increased by 10 %.Among them,the mutation strain LB-5's lipase activity reach 66.4 IU/g,increasing 55.1% of that than original strains. The connerction of the laser radiation power,pulse number and lethal rate were discussed.


 

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