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Construction and Characteristic of a cDNA library from the skin of alpaca
Jiang Jun-bing，Zhang Qiao-ling，Geng Jian-jun，Dong Chang-sheng*
(College of Animal Science and Veterinary Medicine, Shanxi Agricμl tural University, Taigu 030801, Shanxi province,China)
Abstract: A cDNA library from the skin of alpaca in China was constructed using the prokaryotic expression vector pet-32-a+. All the cDNA fragments are normalized by 1% Agarose gel, and the fragments below 400 bp were discarded. The digested vector was processed by dephosphorylase and the cDNA fragments were processed by phosphorylase. The positive recombination rate was 100% examined by blue/white colony. The library capacity was 2.25×107. RandomLy selected 7 positive clones were sequenced by Shaihai Sangon bio-engineering company using T7 terminator primer. Two ESTs related to the wool growth were obtained, which are s100 calcium binding A3 gene and high sμl fur keratin-associated protein gene.
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