杨小红1 , 刘承宜2 , 刘少杰1 , 谭见容1 , 梁佩红1
（1. 暨南大学医学院第四附属医院, 广州市创伤外科研究所， 广东 广州510220；
2. 华南师范大学激光运动医学实验室，广东 广州 510631）
摘 要: 目的 了解低强度激光照射对软骨细胞增殖的影响及其机制。方法 选取3周龄新西兰白兔分离培养软骨细胞，在2.5 %新生牛血清中培养，用半导体激光（650 nm，2.96 mW/cm2）（semiconductor laser irradiation, SLI）照第4代软骨细胞后，每天分别照射1 min、3 min、5 min、7 min、10 min、20 min，共6 d。收集激光照射后第2 d、4 d、6 d、8 d、10 d和12 d的细胞培养液，用氯胺T消化法检测羟脯氨酸（Hrp）的含量。在培养至第13 d时，用XTT法检测细胞的活性，了解细胞的增殖情况。结果 在2.5 %新生牛血清中，SLI对软骨细胞具有明显的光生物调节作用：（1）在培养至第13 d时，所有剂量组在照射后XTT吸光度值均有不同程度的增高，其中3 min、5 min、7 min和10 min组的增高较为明显（P<0.01）；（2）两因素重复测定资料的方差分析结果显示，SLI照射后软骨细胞合成胶原的能力在逐步增加，而对照组在培养至第2周开始Hrp含量明显下降。结论：SLI照射可促进2.5 %新生牛血清中兔软骨细胞增殖，这个过程可能是通过促进胶原合成实现的。
|
|
Collagen Synthesis Mediated Chondrocyte Photobiomodulation in Vitro
YANG Xiao-Hong1, LIU Timon Cheng-Yi2, LIU Shao-Jie1, TAN Jian-Rong1, LIANG Pie-Hong1.
( 1. Guangzhou Institute of Traumatology, The 4th Affiliated Hospital of Medical College of Jinan University, Guangzhou 510220, Guangdong, China; 2. Laboratory of Laser Sports Medicine, South China Normal University, Guangzhou 510631, Guangdong, China)
Abstract：Objective：the effects of low intensity laser irradiation on the chondrocyte proliferation and its mechanism were studied. Methods: the chondrocytes isolated from the cartilage sample of 3-week-old New Zealand white rabbits were cultured with 10 % newborn calf serum (NCS), irradiated by 650nm semiconductor laser irradiation (SLI) at 2.96 mW/cm2 for 1 min, 3 min, 5 min, 7 min, 10 min and 20 min per day for 6 days, and then incubated till the 13th day at 2.5 % NCS. The type II collagen synthesis was assessed by a hydroxyproline (Hpr) content measurement on the 2nd, 4th, 6th, 8th, 10th and 12th day after the first SLI irradiation, respectively. The proliferation on the 13th day was assessed by a XTT assay. Results There is significant photobiomodulation on the proliferation of the chondrocytes cultured at 2.5% NCS. (1) The chondrocyte proliferation was significantly (P<0.01) promoted for the groups irradiated by SLI for 3, 5, 7 and 10 min, respectively, on the 13rd day；(2) The type II collagen synthesis increased steadily with days in the group irradiated by SLI for 5 min. Conclusions: the proliferation of the chondrocyte cultured at 2.5 % NCS might be promoted by SLI, which might be mediated by collagen synthesis.
|
