叶 辉1 , 程备久2* , 朱苏文2 , 甘德芳3
（1.安徽农业大学生物技术中心，安徽 合肥 230036；2.安徽农业大学生命科学学院，安徽合肥 230036；3.安徽农业大学园艺学院，安徽 合肥 230036）
摘 要：采用改进的方法提取粘质沙雷氏菌基因组DNA，通过PCR扩增，得到大小为1500 bp特异性DNA片段（chiB基因），以pUC18质粒构建了pUC-chiB克隆载体，转化至感受态细胞E.coli DH5α培养，并筛选出重组质粒。经测序分析，证明克隆片段与文献报道相一致。
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Cloning and Sequence Analysis of Serratia marcescens Chitinase chiB Gene
YE Hui1, CHENG Bei-jiu2*, ZHU Su-wen2, GAN De-fang3
(1. Biotechnology Center of Anhui Agricultural University, Hefei 230036, Anhui, China;
2.School of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, China;
3.School of Horticulture, Anhui Agricultural University, Hefei 230036, Anhui, China;)
Abstract: The genome DNA was extracted from Serratia marcescens by improved method. A special fragment about 1500bp length was cloned from Serratia marcescens genome DNA by Polymerase Chain Reaction(PCR) amplification. Vector pUC-chiB was constructed through ligating the fragment into the plasmid pUC18 and transformed into E.coli DH5α. Through screening of recombinants and sequence analysis of it, the result showed that the cloned DNA fragment was same to the reported.
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