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Sequence Analysis of Sry and Six Dmrt Genes in Neophocaena phocaenoides
CAO Cheng-he 1, ZHENG Bang-you2, MI Li2, JIANG Wen-hua2, NIE Liu-wang1
(1. Life Science College of Anhui Normal University, Anhui Provincial Key Lab of the Conservation and Exploitation of Biological Resources, Wuhu 241000, Anhui，China；2. The River Dolphin National Reserve of Tongling, Tongling 244000, Anhui，China）
Abstract: The Sry(sex determining region on Y chromosome)gene is master switch in sex determining of mammal. The function is start sex determination and differentiation up through work upon Dmrt、Sox3、Sox9 and so on. The Dmrt genes constitute a new gene family related to sex-determination. They encoded transcription factors characterized by a conserved zinc finger-like DNA-binding motif, the DM domain. In this study,the PCR technique was used to amplify the Sry complete sequence and the conserved DM domains of Dmrt genes in Neophocaena phocaenoides.then ,a sequence (760bp) was identified and named as Npsry in male, six different DM sequences（140bp）were identified and named as NpDmrt1,NpDmrt1a, NpDmrt3,NpDmrt3a,NpDmrt4.and NpDmrt5 according to its name Neophocaena phocaenoides. The results indicated that Sry gene and Dmrt gene were all highly conservative in phylogeny ,and the Dmrt genes have several members.
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