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氩离子激光照射对类球红细菌的诱变效应及对辅酶Q10产生量的影响
激光生物学报摘要 2007-3
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武标1 , 张千1 , 李辉1 , 武威2
（1.辽宁大学, 生命科学学院1a ;资源与环境学院1b, 辽宁 沈阳 110036）
摘 要：以低产量辅酶Q10类球红细菌为亲本，以氩离子激光为诱变源，对其幅照诱变，结果发现：亲本株发生了明显的诱变效应，出现了不同的色素突变表型。诱变后的色素突变株不仅遗传性状稳定，且辅酶Q10产量比亲本株有明显提高。对其中的黄色突变株发酵液进行辅酶Q10提取及测定，结果显示：其辅酶Q10产量比亲本株提高102.10％，经发酵条件初步优化，其最高产量可达26.39mg/l发酵液。
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Mutagenic Effect of Ar+-laser Radiation on Rhodobacter Sphaeroides and the Production of Coenzyme Q10
WU Biao1 , ZHANG Qian1 LI Hui1 WU Wei2
(1. Liaoning University，College of Life Science1a ; College of Environment and Resources1b，Shenyang 110036, Liaoning , Liaoning)
Abstract: To study the mutagenic effects of Ar+-laser radiation on Rhodobacter Sphaeroides and the production of Coenzyme Q10(CoQ10).The result showed that:The parent strain generated obvious mutagenic action after Ar+-laser radiation and expressed various obvious phenotypes of pigment mutant.The CoQ10 production of pigment mutants were higher than the original one,an the best production of CoQ10 reached 26.39mg/L under the optimized shake flask fermentation conditions.
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Bioinformatics,
sequence analysis; GCG; Life Science News; Drug Discovery.
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