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树 莓 组 织 培 养 及 植 株 再生
激光生物学报摘要 2007-3
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袁 艺, 李 纯, 张 扬, 谷小飞, 高学玲, 江家声
(安徽农业大学生命科学学院,安徽 合肥 230036）
摘 要：以黑莓沙泥芽为外植体，筛选培养基，建立黑莓离体再生体系。结果表明，诱导树莓侧芽的最适起始培养基为MS+6-BA 1.0 mg/+NAA 0.02+GA 6 mg/L；芽的增殖的最适培养基为MS+GA 6 mg/L+NAA 0.02 mg/L+ 6-BA 1.0 ；最适生根培养基为MS+NAA 0.5 mg/L+IBA 0.1 mg/L；试管苗移栽的最适基质为蛭石：珍珠岩：营养土（1：1：1）。
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Tissue Culture and Plant Regeneration of Rubus ideaus L.
YUAN Yi , LI Chun , ZHANG Yang, GU Xiao-fei, GAO Xue-ling, JIANG Jia-sheng
(College of Life Sciences, Anhui Agriculture University, Hefei 230036, Anhui, China)
Abstract： Using buds and leaves and petiole of Rubus ideaus L as explant, a method for rapid propagation of R.. Ideaus was established. The result showed that the best medium of introduction lateral buds was MS+6-BA 1.0 mg/L+NAA 0.02 mg/L+GA 6 mg/L;the best medium of reproducting of lateral buds was MS+6-BA 1.0 mg/L+NAA 0.02 mg/L+GA 6 mg/L;the best medium of rooting was MS+IBA 0.1 mg/L+NAA 0.5 mg/L. the best medium of callus was MS+6-BA 0.2 mg/L+NAA 1.0 mg/L -1.5 mg/L.
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Bioinformatics,
sequence analysis; GCG; Life Science News; Drug Discovery.
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