王希朝1,2, 段恩奎2
(1.华南师范大学激光声明科学研究所 广东 广州 510631；
2.中国科学院动物研究所计划生育生殖生物学国家重点实验室 北京 100080)
摘 要：目的：种间胚胎移植是挽救濒危动物的一个有效手段。以往的研究主要集中于对种间、同种妊娠之间微观结构和解剖学差异的描述上，而对导致这些现象产生的分子机制的研究却所见甚少。本研究的目的是阐明同种妊娠和科间妊娠之间免疫反应的差异，并初步探讨科间妊娠中流产的可能原因。方法：以科间妊娠D4、D8、D12的孕体为实验组，小鼠同种移植妊娠D4、D8、D12的子宫孕体和相应时间假孕小鼠的子宫为阳性和阴性对照。结果：采用间接免疫荧光和激光共聚焦扫描的方法对冰冻切片显色的结果表明：妊娠D8时，MHCⅡ分子和 Gal-α-Gal 抗原决定簇的表达位置和表达密度在科间妊娠、阳性、阴性对照之间均不相同。妊娠D8时，MHCⅡ分子在科间妊娠中的表达弱于同种妊娠，其表达模式在D12时也不相同。Gal-α-Gal抗原决定簇在科间妊娠D8时不表达，但在同种妊娠中却有一定水平的表达。在妊娠D12，科间妊娠中有一定量的表达，但与同种妊娠的表达模式不同。结论：这些结果表明，科间妊娠和同种妊娠的免疫反应有差异，这些差异可能是造成科间妊娠失败的原因之一。
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Expressions of MHCⅡ and Gal-α-Gal in Interfamily Pregnancy between Golden Hamster and Mouse by Laser Confocal Scan
WANG Xi-chao1,2 ， DUAN En-kui 2
(1. MOE Key Libratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631；2. State Key Laboratory of Reproductive Biology, Institute of Zoology,
Chinese Academy of Sciences, Beijing, 100080)
Abstract: Sbujective: Interspecific embryo transfer could be a valuable tool in preservation programs of endangered species. The majority of previous studies focused on descriptions of microstructure and anatomic differences between intraspecific and interspecific pregnancy, while the report for molecular mechanism of these phenomena was few. The aim of this work was to illuminate the difference of immunological reaction between intraspecific and interfamily pregnancy and to primarily grope for the possible reasons of interfamily pregnant abortion. Methods: The fetuses of interfamily pregnancy day 4, day 8 and day 12 were acted as experimental group, while the ones of intraspcific pregnancy day 4, day 8 and day 12 of embryo transfer and the uteri of pseudopregnant mice of corresponding time were employed as positive and negative groups respectively. Results: The indirect immunofluorescence and laser confocal scan results of cryosections showed: Expressive positions and intensities of MHC class Ⅱ molecules and Gal-α-Gal epitopes were all different in interfamily pregnancy, positive and negative controls in pregnancy day 8.The expressions of MHC class Ⅱ molecules in interfamily pregnancy were weaker than in intraspecfic pregnancy in day 8, and expressive modes in day 12 were also different. Gal-α-Gal epitopes did not expresse in interfamily pregnancy day 8, but in intraspecific pregnancy they expressed in a certain level. In pregnancy day 12, they expressed in a certain degree in interfamily pregnancy, but their expressive modes were different to those in intraspecific pregnancy. Conclusion: These results indicated the differences between interfamily and intraspecific pregnancy. It was possible that these different expressions of MHC class Ⅱ molecules and Gal-α-Gal epitopes would be one of reasons leading to the failure in interfamily pregnancy.
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