Abstract: Bacillus megaterium BM279 was the mutant strain induced by ion bean implantation from BM80. The bio-effects on the physiological and chemical level between BM279 and BM80 were discussed in this study. Ion beam implantation acted no effect on the growth of BM279 itself and exhibited the accordant growth curve with BM80. However, alkaline metabolism secreted by BM279 accelerated the proliferation of GO29 and facilitated to sustain the pH environment for GO29. The 42-h cultivated extra cellular active element from BM279 improved the acid-producing capacity of GO29 than that of BM80. Additionally, this active element from BM279 was excreted 6-h later than that of BM80. The SDH simulative protein SSPBM80 and SSPBM279 from the extra cellular supernatant of BM80 and BM279 were purified by three chromatographic steps respectively. The specific activity of SSPBM279 was 50 percent higher than that of SSPBM80, and which subsequently accelerated the SDH activity of GO29.