激光生物学报摘要, 更新时间: 2007年10月13日
  由美国生科集团 (BVTech, Inc.) 主办
  
天麻多糖提取纯化方法及性质的初步研究
激光生物学报摘要 2007-4

刘玉潭，梁 华，蔡永萍，林 毅，詹少华 （安徽农业大学生命科学学院，安徽 合肥230036）

摘 要：目的：探讨天麻多糖的最佳提取、纯化方法，分析天麻多糖性质，为深入研究天麻多糖提供技术方法。方法：采用水提醇沉法提取出水溶性粗多糖，经Sevag法脱蛋白和活性炭去色素，过DEAE-纤维素柱，盐洗脱得到天麻多糖。进行天麻多糖理化性质分析、紫外光谱和红外光谱检测分析。结果：以10 %乙醇50 ℃恒温回流提取2 h，重复三次，是天麻多糖提取的最佳方法。天麻多糖为白色粉末状固体，其水溶液呈透明粘稠状，pH弱酸性。结论：天麻多糖为非淀粉类非还原性多糖，可能是含有α-D-木吡喃糖、D-吡喃葡萄糖等单糖的非均一组分构成的多糖。


Primary Research on Isolation，Purification and Character of Polysaccharide of Gastrodia elata BL

LIU Yu-tan , LIANG Hua , CAI Yong-ping , LIN Yi , ZHAN Shao-hua (School of Life Sciences , Anhui Agriculture University , Hefei 230036，Anhui，China )

Abstract: Objective: To explore the best purification method and analysis characters polysaccharide of Gastrodia elata BL., in order to provide the technical method for the research on Polysaccharide of Gastrodia elata BL. Methods: First，the water-soluble of crude polysaccharide were obtained by water extraction and ethanol precipitation; second removed albumen by Savage way and pigment by active carbon; third the polysaccharide of Gastrodia elata BL. was obtained after the salt elutes through passing DEAE-cellulose column


 

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