陈同生*, 王龙祥，孙 磊，王会营，邢 达
（华南师范大学激光生命科学研究所，暨激光生命科学教育部重点实验室， 广东 广州510631）
摘 要：消癌平是从萝摩科（asclepiadacease）植物通关藤（marsdenia tenacissima）根部提取的药物，已经广泛应用于癌症和多种炎症的临床治疗，并且具有较好的疗效。将消癌平注射液与细胞培养液按照1:1的比例混合后共同培养人肺腺癌（ASTC-a-1）细胞，通过CCK-8方法检测发现消癌平能够显著抑制细胞的增长。然后利用荧光共聚焦扫描显微镜和荧光共振能量转移（fluorescence resonance energy transfer, FRET）技术在稳定表达了SCAT3质粒的单个ASTC-a-1细胞中实时动态监测消癌平处理后SCAT3的空间分布以及SCAT3被caspase-3切割的动态过程。实验结果表明：消癌平处理后20 min中SCAT3开始向细胞核以及细胞膜部位转移，约在100 min左右SCAT3被迅速切割。
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Monitoring the Dynamics of Caspase-3 Activation Induced by Xiaoaiping
CHEN Tong-sheng*, WANG Long-xiang, SUN Lei, WANG Hui-ying, XING Da
(MOE Key Libratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China)
Abstract: Xiaoaiping (XAP) isolated from the stems of marsdenia tenacissima, an asclepiadacease plant, has been documented to possess functions against inflammation and cancers. In this paper, we used the CCK-8 to measure the cell activity after XAP treatment according to the 1:1 of XAP and DMEM, and we found that XAP inhibited obviously the cell activity. We used confocal fluorescence microscope and fluorescence resonance energy transfer (FRET) to dynamic temporal-spatial distribution of SCAT3 and caspase-3 activation in single living human lung adenocarcinoma line (ASTC-a-1) cell expressing stably with the SCAT3. Experimental results showed that SCAT3 translocated from cytoplasm to nuclear, cell membrane and intracellular membrane area after 20min of XAP treatment, and the caspase-3 was activated rapidly about 100min of XAP treatment.
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