激光生物学报摘要, 更新时间: 2007年11月11日
  由美国生科集团 (BVTech, Inc.) 主办
  
柳蚕卵黄原蛋白cDNA的克隆及序列分析
激光生物学报摘要 2007-5

尹志亮，刘朝良*，邵引刚，李 锋，魏国清，穆 莉，孙 辉，钱 岑 （安徽农业大学生命科学院特种经济动物科学系，安徽 合肥 230036）

摘 要：经RT-PCR和 RACE合成了柳蚕卵黄原蛋白（Vg）cDNA，经序列分析其长度为5701个碱基，由一个ORF组成，编码了1774个氨基酸。氨基酸序列中有2个保守的多聚丝氨酸区域。并推测氨基酸序列中有8个天冬酰胺连接的糖基化位点（N-linked glycosylation sites）。与樟蚕、天蚕、柞蚕、野桑蚕、家蚕、樗蚕、蓖麻蚕的Vg cDNA序列比对后发现有很高的同源性，分别为83.1%、81.1%、81.0%、64.7%、64.6%、79.7%、79.2%，同其它昆虫Vg cDNA序列也具有很高的同源性.根据7种蚕（家蚕、天蚕、柞蚕、蓖麻蚕、樟蚕、野桑蚕、樗蚕）Vg cDNA序列建立了系统发生树。


Clone and Analysis of cDNA of Vitellogenin from Actias selene Hubner

YIN Zhi-liang，LIU Chao-liang, SHAO Yin-gang, LI Feng, WEI Guo-qing, MU Li, SUN Hui, QIAN Cen (College of Life science, Anhui Agriculture University, Hefei 230036)

Abstract: The cDNA sequence of vitellogenin in Actias selene Hubner is obtained through RT-PCR to the total RNA and RACE amplification to 3'-end, which contains 5701 bases, an opening reading frame which encodes 1774 amino acids,composed of 8 N-linked glycosylation sites,2Polyserne regions, Homology of bases between Actias seleneHubner, Saturniajaponica(AB190809), antheraeayamamai(AB055843), Antheraea pernyi(AB049631), Bombyx mandarina(AB055845), Bombyx mori(D13160), Samia Cynthia pryeri(AB190810), Philosamia Cynthia ricini(ab0555844). Homology identities are 83.1%、81.1%、81.0%、64.7%、64.6%、79.7%、79.2% respectively. Homology identity is high compared with other insects.A phylogenetic tree was established based on the vitellogenin cDNA of seven species, including Antheraea.pernyi, antheraea.yamamai, Bombyx.mori. Philosamia Cynthia ricini, Bombyx mandarina, Samia Cynthia pryeri,Saturnia japonica.


 

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