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ZnPcS2P2在K562和HL-60细胞中的定位研究
激光生物学报摘要 2007-5
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陈显凌1，陈元仲1＊，黄慧芳1，陈 荣2，陈小芳1，黄祖芳2
（1.福建医科大学附属协和医院,福建省血液病研究所, 福建 福州350001；2. 福建师范大学物理与光学学院, 福建 福州350007）
摘 要：目的：探讨ZnPcS2P2在K562细胞,HL-60细胞亚细胞结构中的精确定位,揭示光动力学疗法（Photodynamic therapy PDT）的作用机制。方法：将K562细胞,HL-60细胞与ZnPcS2P2共同孵育5 h。应用激光扫描共聚焦显微成像系统，选择特异性细胞器荧光探针(线粒体探针若丹明Rodanmine123、溶酶体探针LysoTracker DND-26、内质网探针Dioc6(3)采用波形比较法对光敏剂进行亚细胞定位。结果：ZnPcS2P2在K562细胞,HL-60细胞中发出的荧光与负载的Rodanmine123、Lyso-TracKer DND-26、Dioc6(3)均有部分重叠，波形均有相似之处。ZnPc-S2P2在线粒体、溶酶体、内质网均有分布。结论：线粒体是ZnPcS2P2介导的PDT(ZnPcS2P2-PDT)光损伤的主要靶点，溶酶体、内质网也是ZnPcS2P2-PDT光损伤的靶点。
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Localizationon of Photosensitizer ZnPcS2P2 on K562 Cells and
HL-60 Cells
CHEN Xian-ling1，CHEN Yuan-zhong1，HUANG Hui-fang1，CHEN Rong2，CHEN Xiao-fang1，HUANG Zu-fang2
（1.Fujian Institute of Hemapathy, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian,China; 2. Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou, Fujian, China）
Objective: To investigate the mechanism of a novel amphipathic photo sensitizer [di-sulfonated di-phthalimidomethyl phthalocyanine zinc (ZnPcS2P2)]-mediated photodynamic therapy (ZnPcS2P2-PDT) on K562 cells and HL60 cells.Methods: Laser scanning confocal microscopy was applied and three special organelle probes including Rhodamine123, Lys -oTracker DND-26 and Dioc6(3) were selected to label mitochondria,lysosome and endoplasmic reticulum fluorescence images both of ZnPc- S2P2 and the three probes were collected through corresponding emission filters. Results: ZnPcS2P2 located on mitochondria lysosome. Endoplasmic reticulum.Conclusion: this study provides direct evidence that mitochondria，lysosome and endoplasmic reticulum play an important role in the apoptosis in K562 cells and HL60 cells induced by ZnPcS2P2 - PDT.
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Bioinformatics,
sequence analysis; GCG; Life Science News; Drug Discovery.
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