激光生物学报摘要, 更新时间: 2007年11月11日
  由美国生科集团 (BVTech, Inc.) 主办
  
猪H-FABP基因多态性与肌内脂肪含量的关联分析
激光生物学报摘要 2007-5

杨文平1 ,曹果清1 ,刘建华1 ,李步高1 ,石建中2 ,周忠孝1 (1. 山西农业大学动物科技学院,中国山西 太谷 030801;2. 山西省大同种猪场,中国山西 大同 037000)

摘 要:以山西白猪为实验材料,采用索氏脂肪抽提法对其背最长肌的肌内脂肪含量进行了测定。根据心脏脂肪酸结合蛋白(Heart fatty acid-binding protein,H-FABP)基因的PCR-RFLP多态性,应用SPSS软件,用最小二乘法拟合一般线性模型(GLM)分析了H-FABP基因的不同位点的基因型对肌内脂肪含量的影响。结果表明,基因型hh、dd、BB对肌内脂肪含量影响的效应值最大。证明H-FABP基因可以作为肌内脂肪含量的侯选基因。


Associations of H-FABP Gene Genetic Polymorphisms with Intramuscular Fat Content in Pigs

YANG Wen-ping1, CAO Guo-qing1,LIU Jian-hua1, LI Bu-gao1,SHI Jian-zhong2,ZHOU Zhong-xiao1 (1. College of Animal Science and Technology , Shanxi Agricultural University , Taigu 030801,Shanxi , China;2. Da Tong Pig Breeding Farm,Datong 037000,Shanxi , China)

Abstract:In this experiment,Shanxi white pigs were used to study the effect of H-FABP gene on intramuscular fat content(IMF%). The m. logissimus over the second to fifth vetebra lumbalis were taken out for intramuscular fat content measurement by Soxhlet’method. Based on H-FABP genetic variation detection,the associations between three PCR-RFLP sites and intramuscular fat content(IMF%)were analyzed by GLM. Results showed that the LSM values of genotype hh,dd and BB of PCR-RFLPs sites were the highest and they had the topmost effect. We suggest that porcine H-FABP gene is worthy of being further studied as candidate gene having effects on IMF%.


 

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