刘嫩容，邱怡申*
（福建师范大学物理与光电信息科技学院 医学光电科学与技术教育部重点实验室，
福建 福州350007）
摘 要：由于生物组织的复杂性和多样性，以及样品制备等因素的影响，实验观察到的生物组织的背向二次谐波（second harmonic generation, SHG）和双光子激发荧光（two-photon excitation fluorescence, TPEF）效应的差异较大。以鼠尾组织作为实验对象，共40个切片，分为横向和纵向、HE染色和未染色四组，采用飞秒激光器作为激发光，用双光子激光扫描共焦显微镜（Two-photon Laser Scanning Confocal Microscope, TPLSCM）观察和分析了样品在不同的制备方式、激发波长、激发功率、扫描深度等条件下的背向SHG和TPEF的变化曲线，讨论和比较了生物组织的背向SHG和TPEF的影响因素以及二者之间的异同，并尝试对实验现象做出了一定的解释。
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Study of Various Effects on Backward SHG and TPEF in Biotissue
LIU Nen-rong，QIU Yi-shen*
(School of Physics and OptoElectronics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007)
Abstract: Because of the complexities and varieties of biological tissues, and the influences of sample preparation, the backward SHG and TPEF of different tissues are different. In this thesis, rat-tails were used as experiment objects, there are 40 slices including transverse and longitudinal, HE-dyed and undyed. Excited by femtosecond laser, the changes of backward SHG and TPEF versus sample preparation, excitation wavelength, excitation power, penetration depth and so on were observed by Two-photon Laser Scanning Confocal Microscope(TPLSCM), effect factors of backward SHG and TPEF and differences of both were discussed and compared, and related comments were also presented.
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