项 艳a，吴大强a，江海洋b，马 庆b，朱苏文b，程备久b
（安徽农业大学 a.林学与园林学院；b. 生命科学学院，安徽 合肥 230036）
摘 要：选用了生产上广泛应用的10个玉米优良自交系，用幼胚通过组织培养研究其再生特性，结果表明玉米自交系基因型间的培养能力有较大的差异，自交系178的再生率高达78 %。在此基础上以其中的178玉米优良自交系为材料，进一步研究了影响玉米成熟胚再生的各种因素，结果表明：高浓度的2,4-二氯苯氧乙酸(2,4-D) (4.0 mg/L)是诱导愈伤组织必须的；在继代培养基中添加适量的2,4-D (2.0 mg/L)、6-苄基嘌呤(6-BA) (0.2 mg/L)和硝酸银(10 mg/L)显著增加胚性愈伤组织的形成；在分化培养中添加0.5 mg/L 6-BA有利于提高愈伤组织的分化频率。这个再生体系的建立，为进一步以成熟胚为受体系统的遗传转化体系的建立奠定了基础。
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Establishment of Plant Regeneration System from Mature Embryos of Maize Elite Inbred lines
XIANG Yan 1, WU Da-Xiang1, JIANG Hai-Yang 2, MA Qing2 , ZHU Su-wen2 ,CHENG Bei-jiu2
( 1.College of Forest and Landscape, Anhui Agricultural University, Hefei 230036,China ; 2. College of Life Science, Anhui Agricultural University,Hefei 230036 ,China )
Abstract: A total of ten widely cultivated maize elite inbred lines were researched in regeneration abilities. Primary callus were easily induced in ten maize cultivars, but the frequencies of embryogenesis callus were different. The results showed maize elite inbred lines 178 had been successfully regenerated and the frequencies were 78 %.An efficient maize regeneration system was developed using mature embryos. Some factors affecting plant regeneration from mature embryos were investigated. The results showed that medium including 4.0 mg/L 2, 4-D was capable to producing primary callus. Among different concentrations of phytohormone, 2.0 mg/L 2, 4-D in combination of 0.2 mg/L BA and 10 mg/L silver nitrate produced the best results, which promoted the formation of embryogenic callus in the subculture medium. And 0.5 mg/L 6-BA has significantly increased the frequency of plant regeneration in the regeneration medium. Using this system, the plantlets were regenerated from maize elite inbred lines 178, providing a powerful basis for genetic transformation of maize.
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