ID EU754723; SV 1; circular; other DNA; STD; SYN; 5238 BP. AC EU754723; DT 04-AUG-2008 (Rel. 96, Created) DT 04-AUG-2008 (Rel. 96, Last updated, Version 1) DE Luciferase ICE T7 Control vector, complete sequence. KW . OS Luciferase ICE T7 control vector OC other sequences; artificial sequences; vectors. RN [1] RP 1-5238 RA Leippe D., Schenborn E., Hartnett J.; RT "TNT(r) T7 Insect Cell Extract Protein Expression System Technical Manual RT TM305 (2008)"; RL Unpublished. RN [2] RP 1-5238 RA Schenborn E., Leippe D.; RT ; RL Submitted (27-MAY-2008) to the EMBL/GenBank/DDBJ databases. RL Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., RL Madison, WI 53711, USA FH Key Location/Qualifiers FT source 1..5238 FT /organism="Luciferase ICE T7 control vector" FT /mol_type="other DNA" FT /db_xref="taxon:551094" FT promoter 21..39 FT /note="T7 RNA polymerase" FT 5'UTR 40..85 FT misc_feature 86..93 FT /note="SgfI site" FT CDS 95..1750 FT /codon_start=1 FT /transl_table=11 FT /product="luciferase" FT /note="firefly luciferase" FT /protein_id="ACF93193.1" FT /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA FT HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP FT ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS FT MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA FT RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK FT IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY FT GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS FT GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL FT LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FT FVDEVPKGLTGKLDARKIREILIKAKKGGKSKLV" FT misc_feature 1745..1752 FT /note="PmeI site" FT 3'UTR 1901..2274 FT repeat_region 2279..2308 FT /rpt_unit_seq="a" FT /note="synthetic" FT terminator 2309..2356 FT CDS 2737..3597 FT /codon_start=1 FT /transl_table=11 FT /product="beta-lactamase" FT /note="amp resistance protein" FT /protein_id="ACF93194.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT rep_origin 3752..3788 FT misc_feature 4459..4744 FT /note="cer site" SQ Sequence 5238 BP; 1420 A; 1176 C; 1275 G; 1367 T; 0 other; ggatctcgat cccgcgaaat taatacgact cactatagga gtatttttat tctttcgtaa 60 aaaaattaga aaaataaaat ataaagcgat cgccatggaa gacgccaaaa acataaagaa 120 aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac tgcataaggc 180 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt 240 gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg 300 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt 360 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta 420 taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag tgtttgtttc 480 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat 540 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac 600 atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct ttgatcgtga 660 caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta agggtgtggc 720 ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta tttttggcaa 780 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat 840 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga 900 agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt tgctagtacc 960 aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt 1020 acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa 1080 acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta catcagctat 1140 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt 1200 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc agagaggcga 1260 attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa 1320 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga 1380 cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag gatatcaggt 1440 ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg acgcgggcgt 1500 ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca 1560 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc 1620 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa 1680 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagtccaa 1740 attggtttaa acgaattcgg gctcggtacc cggggatcct ctagagtcga cctgcaggca 1800 tgcaagctga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg 1860 ctgagcaata actagcataa cctagagtcg ggcggctgta aaacacgata cattgttatt 1920 agtacattta ttaagcgcta gattctgtgc gttgttgatt tacagacaat tgttgtacgt 1980 attttaataa ttcattaaat ttataatctt tagggtggta tgttagagcg aaaatcaaat 2040 gattttcagc gtctttatat ctgaatttaa atattaaatc ctcaatagat ttgtaaaata 2100 ggtttcgatt agtttcaaac aagggttgtt tttccgaacc gatggctgga ctatctaatg 2160 gattttcgct caacgccaca aaacttgcca aatcttgtag cagcaatcta gctttgtcga 2220 tattcgtttg tgttttgttt tgtaataaag gttcgacgtc gttcaaaata ttatccgcaa 2280 aaaaaaaaaa aaaaaaaaaa aaaaaaaact agcataaccc cttggggcct ctaaacgggt 2340 cttgaggggt tttttgctcg cgagcggccg caggcctcta aacgggtctt gaggggtttt 2400 ttgctgaaag gaggaactat atccggttcg cttgctgtcc ataaaaccgc ccagtctagc 2460 tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt 2520 gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct 2580 ttctacgtaa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 2640 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 2700 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 2760 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 2820 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 2880 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 2940 actttcaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 3000 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 3060 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 3120 gataacactg cggccaactt acttctgaca actatcggag gaccgaagga gctaaccgct 3180 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 3240 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 3300 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 3360 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 3420 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 3480 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 3540 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaattc 3600 gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt 3660 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 3720 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 3780 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 3840 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 3900 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 3960 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 4020 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 4080 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 4140 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 4200 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 4260 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 4320 cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc 4380 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 4440 cctagatcct tttatagtcc ggaaatacag gaacgcacgc tggatggccc ttcgctggga 4500 tggtgaaacc atgaaaaatg gcagcttcag tggattaagt gggggtaatg tggcctgtac 4560 cctctggttg cataggtatt catacggtta aaatttatca ggcgcgattg cggcagtttt 4620 tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgctga acgcgtttta 4680 gcggtgcgta caattaaggg attatggtaa atccacttac tgtctgccct cgtagccatc 4740 gagataaacc gcagtactcc ggccacgatg cgtccggcgt agaggatcga gatcttttca 4800 gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg 4860 gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg 4920 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa 4980 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 5040 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga 5100 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc 5160 ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc 5220 aaatatgtat ccgctcat 5238