ID EU855182; SV 1; circular; other DNA; STD; SYN; 11342 BP. AC EU855182; DT 03-AUG-2008 (Rel. 96, Created) DT 03-AUG-2008 (Rel. 96, Last updated, Version 1) DE Cloning vector pAW8_tdTomato, complete sequence. KW . OS Cloning vector pAW8_tdTomato OC other sequences; artificial sequences; vectors. RN [1] RP 1-11342 RX DOI; 10.1016/j.gene.2007.09.024. RX PUBMED; 18054176. RA Watson A.T., Garcia V., Bone N., Carr A.M., Armstrong J.; RT "Gene tagging and gene replacement using recombinase-mediated cassette RT exchange in Schizosaccharomyces pombe"; RL Gene 407(1-2):63-74(2008). RN [2] RP 1-11342 RA Watson A.T.; RT ; RL Submitted (27-JUN-2008) to the EMBL/GenBank/DDBJ databases. RL Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, RL East Sussex BN1 9RQ, UK FH Key Location/Qualifiers FT source 1..11342 FT /organism="Cloning vector pAW8_tdTomato" FT /mol_type="other DNA" FT /db_xref="taxon:550445" FT misc_feature 1..3873 FT /note="Cre-expression plasmid pAW8 backbone" FT misc_recomb 3874..3907 FT /note="loxP" FT CDS 3916..5346 FT /codon_start=1 FT /transl_table=11 FT /product="tandem-dimer red fluorescent protein" FT /note="tdTomato; engineered variant of GenBank Accession FT Number AF506025; N- and C-termini replaced with equivalent FT residues from EGFP" FT /protein_id="ACF75947.1" FT /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA FT KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG FT LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH FT QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH FT HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY FT EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM FT NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV FT LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE FT RSEGRHHLFLYGMDELYK" FT terminator 5355..5583 FT /note="Saccharomyces cerevisiae ADH1" FT gene 5973..6782 FT /gene="kanR" FT CDS 5973..6782 FT /codon_start=1 FT /transl_table=11 FT /gene="kanR" FT /product="kanamycin and geneticin G418 resistance protein" FT /protein_id="ACF75948.1" FT /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP FT DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FT FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FT FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD FT RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT misc_recomb 7030..7063 FT /note="loxM3" FT misc_feature 7064..11342 FT /note="Cre-expression plasmid pAW8 backbone" SQ Sequence 11342 BP; 3099 A; 2604 C; 2503 G; 3136 T; 0 other; caaaaagtta ggtgtaacag aaaatcatga aactatgatt tctaatttat atattggagg 60 attttctcta aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg accatttgat 120 ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa 180 ttttactctg tcagaaacgg ccttaacgac gtagtcgatc cggacgatgg ccaaccttcc 240 aattcattaa atcgagtcct ccatcagatg atttacaagg ttttgcatag aatccctaag 300 taagagaagt tgttagtgat ttcttcaagt ttactaaaat ttacatacaa atggatgatc 360 tcttctccat tgtttccttt cttcttgcaa acgcgtttta caaagagatg acatgctatt 420 ttctgattat ttttttctat agttttctat tttgtacgtc cttgggagtc cgaaatgtaa 480 aatcggggta tggttagtag cggcagcgtt cattatggta tataaaagat aaaaagtagg 540 ggcaagcgag acagaatggg atacaagggc atcgtctata attatagcta aaaattgtat 600 tttaatttgt attttttgta attttatttc tcattgtttt acttaaaatg aatgcgaatt 660 agagaaaact tcaacgaaat gtcaaaataa gctcagcaaa atatacaatt ttagggaaag 720 cgatcagcaa cttattctcc ggtgtagatt ctttttcttc ataaattcca acaatgtaac 780 tgccaattga tttttctcta gatgctaata aattgaaact aatgaatata ataacacagg 840 ttttgataaa ttgtaaactc ttactcaaaa aaagaatgtt aaaactaatt taaccttttt 900 ataaaggctc atggcgttaa agaggatagt aaaaattcta caatttttta atttaatgtc 960 acaattgtct tgcgaatata attctattaa ttttcgctaa ctgctgtttt agtactatta 1020 ctttcaatat gccttatatg caactttaat tcatgaagaa tgcaatattt ttgcaatctt 1080 tcttgtactg agcgcttttt atcatttagt actcattatt taatttttca gtaaagggca 1140 ataaataaat ttgtagaaga tgcaatgtaa tctcctctat ccttttgctc atatgtttat 1200 gagtatacct agtctagaaa ggcttgtatt taaaatatta ttcaataaaa attcacaatt 1260 tttacgacat gtgctaccat tcacttaact tcctgattat aaaattggtt cgtttatact 1320 aattacttaa gtacctttaa ctaaacaaaa tgcctatata tatattaatt tacaatgagt 1380 gtcagataag tcactatgtc cgagtggtta aggagttaga ctcgccatcg atggtatggt 1440 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 1500 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 1560 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 1620 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 1680 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 1740 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1800 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1860 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1920 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1980 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2040 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 2100 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2160 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2220 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2280 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2340 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2400 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2460 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2520 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2580 cccgtatcgt agttatctac acgacgggga gtcaggccac tatggatgaa cgaaatagac 2640 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2700 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2760 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2820 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2880 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2940 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3000 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3060 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3120 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3180 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3240 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3300 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3360 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3420 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3480 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3540 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3600 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 3660 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 3720 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 3780 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 3840 accatgatta cgccaagctt gcatggcggc cgcataactt cgtatagcat acattatacg 3900 aagttatgca tgcttatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc 3960 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag 4020 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg 4080 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag 4140 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag 4200 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag 4260 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc 4320 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac 4380 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg 4440 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac 4500 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac 4560 gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc 4620 ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg 4680 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag 4740 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc 4800 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac 4860 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag 4920 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc 4980 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac 5040 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc 5100 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccgc 5160 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac 5220 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa 5280 cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac 5340 aagtaaggcg cgccacttct aaataagcga atttcttatg atttatgatt tttattatta 5400 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac 5460 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagt 5520 atgaggtcgc tcttattgac cacacctcta ccggcagatc cgctagggat aacagggtaa 5580 tatagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc 5640 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc 5700 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc 5760 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct 5820 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt 5880 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac 5940 agttctcaca tcacatccga acataaacaa ccatgggtaa ggaaaagact cacgtttcga 6000 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 6060 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt 6120 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 6180 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 6240 atgatgcatg gttactcacc actgcgatcc ccggcaaaac agcattccag gtattagaag 6300 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 6360 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 6420 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 6480 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt 6540 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 6600 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 6660 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 6720 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 6780 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt 6840 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct 6900 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg 6960 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtcga 7020 cgaactagta taacttcgta tatggtatta tatacgaagt tatgcggccg cgcgaattca 7080 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 7140 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 7200 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt 7260 acgcatctgt gcggtatttc acaccgcata ccatcgtccg gatcaatgaa gtagatgagt 7320 ttactacctg tatatacttt tttttttgtc aaagtcactt ttatggcatt tccatgttga 7380 aggattagcc acagcactaa attttcagcg tatgattgct tttaaatatt taattttcat 7440 cgttttttaa ttaatattca aaacgattta atgccttcta catctgaagt caagctcata 7500 gactcgggtt gggtagctaa aactacatct aacacgaagg ggtatagcct tcttaaagta 7560 tactcatgta taaacgtttt gggttcatat ttttgggcca cgtggtttat aaaaattctt 7620 aactacacca ctcggtatcc cgcacccgtc tacgtttcta cgatttcgag ctaatattga 7680 gtaaagtgat tagcaaaaaa aataaaagta ctcgttgtcg gagatcaaga atttttctat 7740 tatctcatct aaaccacttt ctaaaagcga aaaacaaaat cgtaatatgc agcttgaatg 7800 ggcttccata gtttgaaaga aaaaccctag cagtactggc aagggagaca ttccttttac 7860 ctggctaatc gccatcttcc agcaggcgca ccattgcccc tgtttcacta tccaggttac 7920 ggatatagtt catgacaata tttacattgg tccagccacc agcttgcatg atctccggta 7980 ttgaaactcc agcgcgggcc atatctcgcg cggctccgac acgggcactg tgtccagacc 8040 aggccaggta tctctgacca gagtcatcct tagcgccgta aatcaatcga tgagttgctt 8100 caaaaatccc ttccagggcg cgagttgata gctggctggt ggcagatggc gcggcaacac 8160 cattttttct gacccggcaa aacaggtagt tattcggatc atcagctaca ccagagacgg 8220 aaatccatcg ctcgaccagt ttagttaccc ccaggctaag tgccttctct acacctgcgg 8280 tgctaaccag cgttttcgtt ctgccaatat ggattaacat tctcccaccg tcagtacgtg 8340 agatatcttt aaccctgatc ctggcaattt cggctatacg taacagggtg ttataagcaa 8400 tccccagaaa tgccagatta cgtatatcct ggcagcgatc gctattttcc atgagtgaac 8460 gaacctggtc gaaatcagtg cgttcgaacg ctagagcctg ttttgcacgt tcaccggcat 8520 caacgttttc ttttcggatc cgccgcataa ccagtgaaac agcattgctg tcacttggtc 8580 gtggcagccc ggaccgacga tgaagcatgt ttagctggcc caaatgttgc tggatagttt 8640 ttactgccag accgcgcgcc tgaagatata gaagataatc gcgaacatct tcaggttctg 8700 cgggaaacca tttccggtta ttcaacttgc accatgccgc ccacgaccgg caaacggaca 8760 gaagcatttt ccaggtatgc tcagaaaacg cctggcgatc cctgaacatg tccatcaggt 8820 tcttgcgaac ctcatcactc gttgcatcga ccggtaactt ttagattaac atttgtttaa 8880 atatgaaatt aggcaatgta acatactgca ggcaaatttt ggtgtacggt aagtaaattg 8940 gacatatgat ttaacaaagc gactataagt cagaaagtga gaatgagatt gaaataatta 9000 attcaactta ttcaattgat gatatgccag gattcctctt cctttatctt caatgtttcg 9060 attaacaggt ccattatccg gtttttagtt tcgctgcaca ttgccgaatg acaatcggat 9120 tcctttaagc tttgcttttg ccctgttttc tctccccagt aatcgttggg tgactgaacc 9180 atccagtagc cgagagttac gtttttcctg atttaataaa tatccatatc tatttgtctc 9240 gtggtgtttt acagcaacta accgaaatta ctattttgca tcatccaacc ataaagagac 9300 aacaataatt ctagaaaaca tataacaagt gtttttttct atttacgtct catacgcaaa 9360 gtccacttta ccatgttttg gaaacttgta cccaaccatt catttttcga ttttgtaatg 9420 aggctctttt cttctaacaa tcattgaggt cattatcatg tgtcctcacc tattgtttaa 9480 attgaggctt gcgctattcc caaaattgtg ggtattattt tcatatatca gtatctgcaa 9540 tttcggttca actccggaag ctcggtaccc aactgtggga atactcaggt atcgtaagat 9600 gcaagagttc gaatctctta gcaaccatta ttttttttct ctcaacataa cgagaacaca 9660 caggggcgct atcgcacaga atcaaattcg atgactggaa attttttgtt aatttcagag 9720 gtcgcctgac gcatatacct ttttcaactg aaaaattggg agaaaaagga aaggtgagag 9780 cgccggaacc ggcttttcat atagaataga gaagcgttca tgactaaatg cttgcatcac 9840 aatacttgaa gttgacaata ttatttaagg acctattgtt ttttccaata ggtggttagc 9900 aatcgtctta ctttctaact tttcttacct tttacatttc agcaatatat atatatatat 9960 atatatatat ttcaaggata taccattgta atgtctgccc ctaagaagat cgtcgttttg 10020 ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct taaagctatt 10080 tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg tggtgctgct 10140 atcgatgcta caggtgttcc acttccagat gaggcgctgg aagcctccaa gaaggctgat 10200 gccgttttgt taggtgctgt gggtggtcct aaatggggta ccggtagtgt tagacctgaa 10260 caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag accatgtaac 10320 tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc taaaggtact 10380 gacttcgttg ttgttagaga attagtggga ggtatttact ttggtaagag aaaggaagac 10440 gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt gcaaagaatc 10500 acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat ttggtccttg 10560 gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc 10620 aagaacgaat ttcctacatt gaaagttcaa catcaattga ttgattctgc cgccatgatc 10680 ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat gtttggtgat 10740 atcatctccg atgaagcctc cgttatccca ggctccttgg gtttgttgcc atctgcgtcc 10800 ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg ccatggttcc 10860 gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc tgctgcaatg 10920 atgttgaaat tgtcattgaa cttgcctgaa gaaggtaaag ccattgaaga tgcagttaaa 10980 aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag taccaccgaa 11040 gtcggtgatg ctgtcgccga agaagttaag aaaatccttg cttaaaaaga ttctcttttt 11100 ttgtgatatt tgtacataaa ctttataaat gaaattcata atagaaacga cacgaaatta 11160 caaaatggaa tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc 11220 aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc 11280 aacgtgataa ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcatca 11340 ca 11342