ID EU912545; SV 1; circular; other DNA; STD; SYN; 3666 BP. AC EU912545; DT 11-SEP-2008 (Rel. 97, Created) DT 08-FEB-2009 (Rel. 99, Last updated, Version 2) DE Cloning vector pDM131, complete sequence. KW . OS Cloning vector pDM131 OC other sequences; artificial sequences; vectors. RN [1] RP 1-3666 RX PUBMED; 19063918. RA Veltman D.M., Akar G., Bosgraaf L., Van Haastert P.J.; RT "A new set of small, extrachromosomal expression vectors for Dictyostelium RT discoideum"; RL Plasmid 61(2):110-118(2009). RN [2] RP 1-3666 RA Veltman D.M., Akar G., Bosgraaf L., van Haastert P.J.M.; RT ; RL Submitted (19-JUL-2008) to the EMBL/GenBank/DDBJ databases. RL Cell Biochemistry, Rijks Universiteit Groningen, Kerklaan 30, Haren 9751 RL NN, The Netherlands FH Key Location/Qualifiers FT source 1..3666 FT /organism="Cloning vector pDM131" FT /mol_type="other DNA" FT /note="mRFPmars-tag for N-terminal fusion" FT /db_xref="taxon:555766" FT CDS 12..>686 FT /codon_start=1 FT /transl_table=11 FT /product="monomeric red fluorescent protein mars" FT /protein_id="ACH81570.1" FT /translation="MASSEDVIKEFMRFKVKMEGSVNGHEFEIEGEGEGRPYEGTQTAK FT LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERIMNFEDGGV FT VTVTQDSTLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM FT RLKLKDGGHYDAEVKTTYKAKKQVQLPGAYKTDIKLDIISHNEDYTIVEQYERAEGRHS FT TGA" FT rep_origin complement(1164..1831) FT /note="pUC ori" FT CDS complement(1979..2839) FT /codon_start=1 FT /transl_table=11 FT /product="penicillin beta-lactamase" FT /protein_id="ACH81571.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" SQ Sequence 3666 BP; 987 A; 845 C; 872 G; 962 T; 0 other; ggatccaaaa aatggcatca tcagaagatg ttattaaaga atttatgaga ttcaaagtta 60 aaatggaagg ttcagttaac ggtcatgaat ttgaaattga aggtgaaggt gaaggtagac 120 catatgaagg tacacaaaca gcaaaattaa aagttacaaa aggtggtcca ttaccatttg 180 catgggatat tctttcacca caatttcaat atggtagtaa agcatatgtt aaacatccag 240 cagatattcc agattatctt aaactttcat ttccagaagg ttttaaatgg gaaagaatta 300 tgaattttga agatggtggt gttgtaacag ttactcaaga ttcaacatta caagatggtg 360 aatttattta taaagttaaa ttaagaggta ccaattttcc atcagatggt ccagtaatgc 420 aaaaaaaaac tatgggttgg gaagcatcaa cagaaagaat gtatccagaa gatggtgcac 480 taaaaggtga aattaaaatg agattaaaac ttaaggatgg tggtcattat gatgcagaag 540 ttaaaacaac ttataaagct aaaaaacaag ttcaattacc aggtgcttat aaaacagata 600 ttaaattaga tattatttca cataatgaag attatactat tgttgaacaa tatgaaagag 660 cagaaggtag acattcaaca ggtgcaagat ctagtactag tgcatcgaat tcctgcagcc 720 cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc 780 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa 840 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 900 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 960 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 1020 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 1080 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 1140 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 1200 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 1260 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 1320 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 1380 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 1440 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 1500 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 1560 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 1620 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 1680 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 1740 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 1800 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 1860 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 1920 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1980 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 2040 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 2100 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 2160 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 2220 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 2280 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 2340 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 2400 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 2460 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 2520 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 2580 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 2640 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 2700 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 2760 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 2820 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 2880 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 2940 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg 3000 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 3060 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 3120 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 3180 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 3240 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 3300 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 3360 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 3420 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 3480 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 3540 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga 3600 ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct 3660 tgatgc 3666