ID FJ267590; SV 1; circular; other DNA; STD; SYN; 8021 BP. AC FJ267590; DT 14-OCT-2008 (Rel. 97, Created) DT 14-OCT-2008 (Rel. 97, Last updated, Version 1) DE Replicative vector pMO79, complete sequence. KW . OS Replicative vector pMO79 OC other sequences; artificial sequences; vectors. RN [1] RP 1-8021 RX DOI; 10.1371/journal.pone.0001976. RX PUBMED; 18398486. RA Jones-Carson J., Laughlin J., Hamad M.A., Stewart A.L., Voskuil M.I., RA Vazquez-Torres A.; RT "Inactivation of [Fe-S] metalloproteins mediates nitric oxide-dependent RT killing of Burkholderia mallei"; RL PLoS One 3(4):E1976-E1976(2008). RN [2] RP 1-8021 RA Jones-Carson J., Laughlin J., Hamad M.A., Stewart A., Voskuil M., RA Vazquez-Torres A.; RT ; RL Submitted (11-SEP-2008) to the EMBL/GenBank/DDBJ databases. RL Microbiology, UCD, 12800 E. 19th Ave., Denver, CO 80045, USA FH Key Location/Qualifiers FT source 1..8021 FT /organism="Replicative vector pMO79" FT /mol_type="other DNA" FT /db_xref="taxon:565108" FT CDS 201..995 FT /codon_start=1 FT /transl_table=11 FT /product="AphA" FT /note="kanamycin resistance marker" FT /protein_id="ACI42378.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT CDS 1010..1825 FT /codon_start=1 FT /transl_table=11 FT /product="kanamycin resistance protein" FT /protein_id="ACI42379.1" FT /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS 1924..2640 FT /codon_start=1 FT /transl_table=11 FT /product="GFP" FT /protein_id="ACI42380.1" FT /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FT FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG FT NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA FT NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FT FVTAAGITHGMDELYK" FT misc_feature 3542..4561 FT /note="mobilization region" FT rep_origin 5357..5368 FT /note="oriV" FT CDS 5555..6217 FT /codon_start=1 FT /transl_table=11 FT /product="RepA" FT /note="replication protein" FT /protein_id="ACI42376.1" FT /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH FT HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV FT NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE FT PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" FT CDS 7085..7804 FT /codon_start=1 FT /transl_table=11 FT /product="RFP-mcherry" FT /protein_id="ACI42377.1" FT /translation="MRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPF FT AWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQD FT GEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYD FT AEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGASGLRSKLN FT PPDESGPGCMSCKCVLS" SQ Sequence 8021 BP; 1780 A; 2235 C; 2180 G; 1826 T; 0 other; gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 60 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 120 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 180 aatattgaaa aaggaagagt atgattgaac aagatggatt gcacgcaggt tctccggccg 240 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 300 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 360 ccggtgccct gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg 420 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 480 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 540 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 600 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 660 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 720 tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 780 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 840 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 900 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 960 tcgccttcta tcgccttctt gacgagttct tctgattaat taaactagta tgagccatat 1020 tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata 1080 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta 1140 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga 1200 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat 1260 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa 1320 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct 1380 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga 1440 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag 1500 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa 1560 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct 1620 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga 1680 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca 1740 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca 1800 tttgatgctc gatgagtttt tctaattaat taatgttgac tcccttagta atttcggaat 1860 attatgctag gttccggttc ttgacagctt atcatcgata agctataata ggaggaaacg 1920 atgatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat 1980 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac 2040 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca 2100 cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa 2160 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct 2220 ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt 2280 gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac 2340 aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat 2400 ggaatcaaag ctaacttcaa aattcgccac aacattgaag atgggtccgt tcaactagca 2460 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat 2520 tacctctcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc 2580 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa 2640 ccgcggaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga 2700 gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt 2760 ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc 2820 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac 2880 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 2940 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 3000 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 3060 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagcgaga aatcaccatg 3120 agtgacgact gaatccggtg agaatggcaa aagctaaaaa ggccgtaata tccagctgaa 3180 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 3240 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 3300 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat 3360 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc 3420 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg 3480 tcacaggtat ttattcgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat 3540 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgcc 3600 cgcgttcctg ctggcgctgg gcctgtttct ggcgctggac ttcccgctgt tccgtcagca 3660 gcttttcgcc cacggccttg atgatcgcgg cggccttggc ctgcatatcc cgattcaacg 3720 gccccagggc gtccagaacg ggcttcaggc gctcccgaag gtctcgggcc gtctcttggg 3780 cttgatcggc cttcttgcgc atctcacgcg ctcctgcggc ggcctgtagg gcaggctcat 3840 acccctgccg aaccgctttt gtcagccggt cggccacggc ttccggcgtc tcaacgcgct 3900 ttgagattcc cagcttttcg gccaatccct gcggtgcata ggcgcgtggc tcgaccgctt 3960 gcgggctgat ggtgacgtgg cccactggtg gccgctccag ggcctcgtag aacgcctgaa 4020 tgcgcgtgtg acgtgccttg ctgccctcga tgccccgttg cagccctaga tcggccacag 4080 cggccgcaaa cgtggtctgg tcgcgggtca tctgcgcttt gttgccgatg aactccttgg 4140 ccgacagcct gccgtcctgc gtcagcggca ccacgaacgc ggtcatgtgc gggctggttt 4200 cgtcacggtg gatgctggcc gtcacgatgc gatccgcccc gtacttgtcc gccagccact 4260 tgtgcgcctt ctcgaagaac gccgcctgct gttcttggct ggccgacttc caccattccg 4320 ggctggccgt catgacgtac tcgaccgcca acacagcgtc cttgcgccgc ttctctggca 4380 gcaactcgcg cagtcggccc atcgcttcat cggtgctgct ggccgcccag tgctcgttct 4440 ctggcgtcct gctggcgtca gcgttgggcg tctcgcgctc gcggtaggcg tgcttgagac 4500 tggccgccac gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca 4560 tgcctgcccc tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct 4620 ccggcgggcc actcaatgct tgagtatact cactagactt tgcttcgcaa agtcgtgacc 4680 gcctacggcg gctgcggcgc cctacgggct tgctctccgg gcttcgccct gcgcggtcgc 4740 tgcgctccct tgccagcccg tggatatgtg gacgatggcc gcgagcggcc accggctggc 4800 tcgcttcgct cggcccgtgg acaaccctgc tggacaagct gatggacagg ctgcgcctgc 4860 ccacgagctt gaccacaggg attgcccacc ggctacccag ccttcgacca catacccacc 4920 ggctccaact gcgcggcctg cggccttgcc ccatcaattt ttttaatttt ctctggggaa 4980 aagcctccgg cctgcggcct gcgcgcttcg cttgccggtt ggacaccaag tggaaggcgg 5040 gtcaaggctc gcgcagcgac cgcgcagcgg cttggccttg acgcgcctgg aacgacccaa 5100 gcctatgcga gtgggggcag tcgaaggcga agcccgcccg cctgcccccc gagcctcacg 5160 gcggcgagtg cgggggttcc aagggggcag cgccaccttg ggcaaggccg aaggccgcgc 5220 agtcgatcaa caagccccgg aggggccact ttttgccgga gggggagccg cgccgaaggc 5280 gtgggggaac cccgcagggg tgcccttctt tgggcaccaa agaactagat atagggcgaa 5340 atgcgaaaga cttaaaaatc aacaacttaa aaaagggggg tacgcaacag ctcattgcgg 5400 caccccccgc aatagctcat tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt 5460 acgcaacgca taattgttgt cgcgctgccg aaaagttgca gctgattgcg catggtgccg 5520 caaccgtgcg gcaccctacc gcatggagat aagcatggcc acgcagtcca gagaaatcgg 5580 cattcaagcc aagaacaagc ccggtcactg ggtgcaaacg gaacgcaaag cgcatgaggc 5640 gtgggccggg cttattgcga ggaaacccac ggcggcaatg ctgctgcatc acctcgtggc 5700 gcagatgggc caccagaacg ccgtggtggt cagccagaag acactttcca agctcatcgg 5760 acgttctttg cggacggtcc aatacgcagt caaggacttg gtggccgagc gctggatctc 5820 cgtcgtgaag ctcaacggcc ccggcaccgt gtcggcctac gtggtcaatg accgcgtggc 5880 gtggggccag ccccgcgacc agttgcgcct gtcggtgttc agtgccgccg tggtggttga 5940 tcacgacgac caggacgaat cgctgttggg gcatggcgac ctgcgccgca tcccgaccct 6000 gtatccgggc gagcagcaac taccgaccgg ccccggcgag gagccgccca gccagcccgg 6060 cattccgggc atggaaccag acctgccagc cttgaccgaa acggaggaat gggaacggcg 6120 cgggcagcag cgcctgccga tgcccgatga gccgtgtttt ctggacgatg gcgagccgtt 6180 ggagccgccg acacgggtca cgctgccgcg ccggtagcac ttgggttgcg cagcaacccg 6240 taagtgcgct gttccagact atcggctgta gccgcctcgc cgccctatac cttgtctgcc 6300 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg 6360 gcacctcgct aacggattca ccgtttttat caggctctgg gaggcagaat aaatgatcat 6420 atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg catttgagaa 6480 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc 6540 ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg 6600 ccattcatcc gcttattatc acttattcag gcgtagcacc agatatcagg gccccgctag 6660 ccagatctgg tcgactacgt actcgccgca gccgaacgac cgagcgcagc gagtcagtga 6720 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 6780 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 6840 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 6900 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 6960 attacgccaa gcttgcacgc ctgccgctcg actcttgagg acccccgggt accgagctcg 7020 aattcctgtt gactcacttg ggattttcgg aatatcatgc cgggttccgg ttcaggagga 7080 aacgatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga 7140 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa 7200 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa 7260 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg 7320 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga 7380 ctcctccctc caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc 7440 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat 7500 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg 7560 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc 7620 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat 7680 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcgcctccg gactcagatc 7740 taagctgaac cctcctgatg agagtggccc cggctgcatg agctgcaagt gtgtgctctc 7800 ctgacatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 7860 gacacccgcc aacacccgct gacgcggccg ccctgcagcg gatccctcta gatgcatgcg 7920 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg 7980 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a 8021